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Tipo: Artigo de Periódico
Título : Marine bacteria from rocas atoll: a promising biotechnological resource
Autor : Velasco-Alzate, Karen Y.
Bauermeister, Anelize
Tangerina, Marcelo M. P.
Lotufo, Tito Monteiro da Cruz
Ferreira, Marcelo J. P.
Jimenez, Paula C.
Padilla, Gabriel
Lopes, Norberto Peporine
Costa-Lotufo, Letícia V.
Palabras clave en portugués brasileño: Rochas;Diversidade microbiologica;Bactéria Marinha
Palabras clave en inglés: Rocas Atoll;Microbial diversity;Marine bacteria
Fecha de publicación : 2019
Editorial : Marine Drugs
Citación : ALZATE-VELASCO, K. ; BAUERMEISTER, A. ; TANGERINA, M. ; LOTUFO, Tito Monteiro da Cruz; FERREIRA, Marcelo J. P.; JIMENEZ, Paula Christine ; PADILLA, Gabriel ; Lopes, Norberto Peporine ; COSTA-LOTUFO, Letícia V. Marine bacteria from rocas atoll: a promising biotechnological resource. Marine Drugs, Switzerland, v. 17, p. 671, 2019. Disponível em: https://www.mdpi.com/1660-3397/17/12/671.Acesso em: 11 set. 2023.
Abstract: Rocas Atoll is a unique environment in the equatorial Atlantic Ocean, hosting a large number of endemic species, however, studies on the chemical diversity emerging from this biota are rather scarce. Therefore, the present work aims to assess the metabolomic diversity and pharmacological potential of the microbiota from Rocas Atoll. A total of 76 bacteria were isolated and cultured in liquid culture media to obtain crude extracts. About one third (34%) of these extracts were recognized as cytotoxic against human colon adenocarcinoma HCT-116 cell line. 16S rRNA gene sequencing analyses revealed that the bacteria producing cytotoxic extracts were mainly from the Actinobacteria phylum, including Streptomyces, Salinispora, Nocardiopsis, and Brevibacterium genera, and in a smaller proportion from Firmicutes phylum (Bacillus). The search in the spectral library in GNPS (Global Natural Products Social Molecular Networking) unveiled a high chemodiversity being produced by these bacteria, including rifamycins, antimycins, desferrioxamines, ferrioxamines, surfactins, surugamides, staurosporines, and saliniketals, along with several unidentified compounds. Using an original approach, molecular networking successfully highlighted groups of compounds responsible for the cytotoxicity of crude extracts. Application of DEREPLICATOR+ (GNPS) allowed the annotation of macrolide novonestimycin derivatives as the cytotoxic compounds existing in the extracts produced by Streptomyces BRB-298 and BRB-302. Overall, these results highlighted the pharmacological potential of bacteria from this singular atoll.
URI : http://repositorio.ufc.br/handle/riufc/74263
ISSN : 1660-3397
Derechos de acceso: Acesso Aberto
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