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http://repositorio.ufc.br/handle/riufc/74263
Type: | Artigo de Periódico |
Title: | Marine bacteria from rocas atoll: a promising biotechnological resource |
Authors: | Velasco-Alzate, Karen Y. Bauermeister, Anelize Tangerina, Marcelo M. P. Lotufo, Tito Monteiro da Cruz Ferreira, Marcelo J. P. Jimenez, Paula C. Padilla, Gabriel Lopes, Norberto Peporine Costa-Lotufo, Letícia V. |
Keywords in Brazilian Portuguese : | Rochas;Diversidade microbiologica;Bactéria Marinha |
Keywords in English : | Rocas Atoll;Microbial diversity;Marine bacteria |
Issue Date: | 2019 |
Publisher: | Marine Drugs |
Citation: | ALZATE-VELASCO, K. ; BAUERMEISTER, A. ; TANGERINA, M. ; LOTUFO, Tito Monteiro da Cruz; FERREIRA, Marcelo J. P.; JIMENEZ, Paula Christine ; PADILLA, Gabriel ; Lopes, Norberto Peporine ; COSTA-LOTUFO, Letícia V. Marine bacteria from rocas atoll: a promising biotechnological resource. Marine Drugs, Switzerland, v. 17, p. 671, 2019. Disponível em: https://www.mdpi.com/1660-3397/17/12/671.Acesso em: 11 set. 2023. |
Abstract: | Rocas Atoll is a unique environment in the equatorial Atlantic Ocean, hosting a large number of endemic species, however, studies on the chemical diversity emerging from this biota are rather scarce. Therefore, the present work aims to assess the metabolomic diversity and pharmacological potential of the microbiota from Rocas Atoll. A total of 76 bacteria were isolated and cultured in liquid culture media to obtain crude extracts. About one third (34%) of these extracts were recognized as cytotoxic against human colon adenocarcinoma HCT-116 cell line. 16S rRNA gene sequencing analyses revealed that the bacteria producing cytotoxic extracts were mainly from the Actinobacteria phylum, including Streptomyces, Salinispora, Nocardiopsis, and Brevibacterium genera, and in a smaller proportion from Firmicutes phylum (Bacillus). The search in the spectral library in GNPS (Global Natural Products Social Molecular Networking) unveiled a high chemodiversity being produced by these bacteria, including rifamycins, antimycins, desferrioxamines, ferrioxamines, surfactins, surugamides, staurosporines, and saliniketals, along with several unidentified compounds. Using an original approach, molecular networking successfully highlighted groups of compounds responsible for the cytotoxicity of crude extracts. Application of DEREPLICATOR+ (GNPS) allowed the annotation of macrolide novonestimycin derivatives as the cytotoxic compounds existing in the extracts produced by Streptomyces BRB-298 and BRB-302. Overall, these results highlighted the pharmacological potential of bacteria from this singular atoll. |
URI: | http://repositorio.ufc.br/handle/riufc/74263 |
ISSN: | 1660-3397 |
Access Rights: | Acesso Aberto |
Appears in Collections: | LABOMAR - Artigos publicados em revistas científicas |
Files in This Item:
File | Description | Size | Format | |
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2019_art_kyvelascoalzate.pdf | 3,64 MB | Adobe PDF | View/Open |
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