Criação de um modelo de biblioteca de extratos (extratoteca) aplicado a banco ativo de germoplasma de folhas de cajazeira

Abstract

The preservation of genetic resources from biodiversity is essential to maintain life on Earth, in food distribution and medicine. Plantae kingdom produce molecules of interest that results of their metabolism and presents a source of new compounds that is almost endless. A library of extracts is a method to save information of different vegetal samples. Yellow mombin (Spondias mombin) has various proved biological activities. However, literature does not provide a detailed chemical profile for this plant. Samples from an active collection of germplasm of yellow mombin were collected to stablish Embrapa’s first library of extracts including samples from a germplasm collection. A method to stock and track all samples in the collection was developed as well as an in silico database. The chemical identification of S. mombin was carried using Ultra Performance Liquid Chromatography hyphenated with a high-resolution mass spectrometer in positive ion mode (UPLC-QToF-MS/MSE). Hidroethanolic extracts (7:3) of mombim leaves were prepared using ultrasound assisted liquid-liquid extraction. A standard method had been made for this procedure. On the first part of the chromatogram the attempt of identification reveled catequin and chlorogenic acid. The extract seemed to have high levels of phenolic compounds. Two peaks appeared to be ellagitannins, very similar to those previously reported for showing antiviral properties, and some quercetin glycosides that presented typical fragments for this type of molecule. Sespendole, identified in other Anacardiaceae species, was also found in the analyses. All compounds were partially identified. The development of a standard method to improve the library of extracts will result in the increase of the physical and digital database, which represents a positive perspective to this work.