Estudo das correntes de Na+ dependentes de voltagem experimentais e simulados do gânglio cervical superior de ratos diabéticos induzidos por estreptozotocina

Abstract

Diabetes mellitus (DM) is a metabolic disorder with high worldwide prevalence in the adult and elderly population, and is often associated with a series o f organic complications, including changes in the expression and/or function of voltage dependent sodium channels (Nav). The streptozotocin-induced DM model (STZ) has been widely used to study these complications. The objective of this study was to obtain the Nav currents of neurons of the Superior Cervical Ganglion (GCS) of healthy animals and with STZ-induced DM and later compare these with data simulated in a computer program. Regarding methodological aspects, the data of this work come from the project submitted to CEUA/UECE, which received approval under number 080921131/2020. Rattus novergicus of the Wistar variety of both sexes were used, the electrophysiological recordings of the Nav were obtained through the patch clamp technique. The simulations ar e based on a simplified cell model using the Dev-C++ software version 6.30. The data obtained were expressed as mean ± standard error of the mean, and the comparison between groups was performed by Student's t -test. After DM induction, it was possible to verify that there was a reduction in body mass in this group of animals and maintenance of the hyperglycemia condition. Regarding the electrophysiological findings, there was a change in current amplitude, which was smaller for the DM group, which was also confirmed in the evaluation of representative tracings. In the simulation of Na+ currents, we used a C/C++ language program according to Belluzzi and Sacchi equations (1991 and 1998). After making the necessary adjustments to some of the measurements used in the equations, it was possible to simulate the curves and traces found in the experimental groups. It is concluded from this study that the DM model used was effective. Furthermore, it is capable of modifying the electrophysiological parameters of Na v. Finally, the developed simulations provided a good prediction for the Na+ current findings