O uso da sacarose contribui para o desenvolvimento in vitro da batata doce

Abstract

In vitro cultivation is a propagation method of great importance. Because it enables fast and high multiplication capacity, high quality genetic and to be free of pathogens. However, the low availability of CO2, in which plants are submitted, as well as low light intensity, can make this environment inadequate for the performance of photosynthesis by plants, which makes it necessary to use an external source of carbohydrates. Therefore, this study aimed to evaluate the influence of different sucrose concentrations, as a carbohydrate source, on the in vitro development of micropropagated sweet potato seedlings in liquid and solid culture media. The experiment was installed in a completely randomized experimental design and factorial scheme of 2 x 4, being two conditions of culture medium (liquid and solid) and four sucrose concentrations (0, 30, 60, 80 g L-1 ), totaling eight treatments with 12 replicates. After 40 days, the plants were evaluated for phytotechnical characteristics (height, number of leaves, number of shoots, number of roots, number of senescidal leaves, fresh mass, and dry mass). The T6 treatment (30 g L1 sucrose in solid medium) was the one that provided the best results for all variables analyzed, except for the number of roots. In view of the results, it was possible to show that the concentration of 30 g L-1 sucrose in solid culture medium was the best for the development, in vitro, of var Campina sweet potato seedlings.