Determinação do teor total de taninos e avaliação do potencial toxicológico de coprodutos de frutas tropicais comercializadas no Ceará

Abstract

Brazil has great potential for the production of vegetables, and, therefore, is one of the countries that produce more agro-industrial residues that, due to its poor management of utilization, cause serious environmental impacts. About a third of food intended for human consumption is wasted due to different factors, and that they have great application potential due to their significant nutritional and technological potential even after being manipulated, with high levels of macronutrients and micronutrients. Thus, the present work aimed to evaluate the total tannin content and the toxicity of extracts obtained from residues of fruit co-products from the state of Ceará against Artemia salina and Zebrafish. The plant materials used were obtained from the local market in the state of Ceará, as well as the model organisms used. The present study focused on tropical fruit co-products of Carica papaya, Ananas comosus, Passiflora edulis, Psidium guajava, Tamarindus indica, Anacardium occidentale, Artocarpus heterophyllus, Malpighia emarginata, Chrysobalanus icaco L. and Eugenia uniflora L, in which they were dried. using a lyophilizer, extracted with ethanol under heating, filtered, concentrated, conditioned and stored under freezing, for further analysis of quantification of the content of total tannins and in vivo bioassays against Zebrafish and Artêmia salina. The tested extracts, in the bioassay against Artêmia salina, showed a total absence of mortality, showing LC50%>1000µg/mL. In the quantification of total tannins, the acerola extract had the highest content, with 13066.67 g of TAE/100 g of extract, differing statistically from all other plant materials, and they ranged from 5704.76 to 297 .24 g TAE/100 g extract. For the analysis of acute toxicity, at the standard concentration tested of 250 mg/ml, the extracts did not demonstrate toxicity in any sample, which corroborates the results previously obtained in the toxicity bioassay against Artêmia salina, noting the reliability of atoxicity in the present hydroalcoholic extracts. Therefore, in the present study, high levels of total tannins and related toxicity can be observed from tropical fruit co-product extracts tested by bioassay methods against two characteristic model organisms, Artêmia salina and Danio rerio.