Efeito do extrato hidroalcoólico do caule de Cassia fistula linn. (chuva-de-ouro) sobre o processo de cicatrização em camundongos

Abstract

Introduction: The healing process of skin lesions is complex and involves cells and chemical mediators aimed at tissue repair. When these wounds become chronic, treatments are expensive and difficult to access. In this sense, phytotherapy is an alternative to be considered. Cassia Fistula Linn (golden rain) is a plant distributed in the semi-arid region of Brazil and its stem is used in folk medicine for the treatment of wounds and its fruits are marketed in association with other plants (Tamarine® ) as a laxative. Previous studies demonstrated the healing potential of its leaves and flowers and this effect was observed in the hydroalcoholic stem extract (ECFC) in in vitro experiments. Objective: To approach the treatment of excisional wounds in Swiss mice using a cream containing ECFC (CrECFC) at concentrations of 12.5 and 25% w/v. Methods: Healing was evaluated by measuring the rate of lesion contraction on days 2, 4, 7, 9 and 12 after surgery, as well as by histological analysis and dosage of chemical mediators on days 2, 7 and 12. Results: CrECFC increased the rate of contraction of the lesions from the 7th day was able to stimulate the formation of a new epithelium with considerable deposits of keratin that partially covered the ulcer and the development of a remodeled connective tissue. In addition, it decreased the levels of TNF-α and IL-1β after 2 days of treatment and on the 7th day, it was able to increase the levels of TGF-β and VEGF in relation to the group treated with the vehicle alone. Conclusion: In view of these results, we suggest that treatment with CrECFC in animal skin wounds accelerated healing and is capable of driving future clinical studies.