Padronização de um procedimento de canulação crônica intracerebral guiado por estereotaxia

Abstract

The increase in the prevalence of neuropsychopathologies requires a response from science in terms of new research approaches and the construction of novel animal models that help elucidate existing gaps in pathophysiological mechanisms, develop prevention strategies and contribute to pharmacological technologies that obtain better adverse effects, tolerability and substitute pharmacoresistances. The Chronic cannulation intracerebral guided by stereotactic represents a technique with potential for innovation in research and to continue the investigation of the pre-clinical studies already consecrated. This is a strategic tool that allows to reach accurately and safely any area located in the brain in a minimally invasive way, which allows the investigation of the effects of neurotransmitters and signaling pathways in animals agreed, that is, adding the behavioral study to the analyzes. The successful reproduction of this technique, combined with the saving of time, human and financial resources, is possible only with a standardization of the procedures that is submitted to Good Laboratory Practices and to the norms recommended by ABNT ISO / IEC 17025. The Operational Procedures produced in this work aim at standardization of the technique in order to guarantee the quality of the experiments, to ensure the repeatability, reproducibility and traceability of the data, in addition to obtaining more reliable results. Thus, this work acquires importance as a guide for procedures that involve stereotactic and as a reference for the accreditation of research laboratories.