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http://repositorio.ufc.br/handle/riufc/81250| Type: | Artigo de Periódico |
| Title: | Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). |
| Authors: | Carvalho, Edirsana Maria Ribeiro Menezes, Francisca Gleire Rodrigues de Santos, Daniel Rodrigues dos Souza, Oscarina Viana de Hofer, Ernesto Vieira, Regine Helena silva dos Fernandes |
| Keywords in Brazilian Portuguese : | Aquicultura;Antibiótico;Integron |
| Keywords in English : | Aquaculture;Antibiotic;Integron |
| Issue Date: | 2022 |
| Publisher: | Revista Científica Semana Acadêmica |
| Citation: | CARVALHO, Edirsana Maria Ribeiro; MENEZES, Francisca Gleire Rodrigues des; SANTOS, Daniel Rodrigues dos; SOUZA, Oscarina Viana de; HOFER, Ernesto; VIEIRA, Regine Helena Silva dos Fernandes. Integrons and gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (LITOPENAEUS VANNAMEI). Revista Científica Semana Acadêmica, v. 10, p. 1-16, 2022. Disponível em: https://semanaacademica.org.br/artigo/integrons-and-gene-cassettes-encoding-antimicrobial-resistance-vibrios-isolated-shrimp. Acesso em: 11 jun 2025. |
| Abstract in Brazilian Portuguese: | O objetivo do presente estudo foi identificar integrons e cassetes gênicos em vibrios isolados da hemolinfa de camarão (Litopenaeus vannamei) cultivado no Ceará (Nordeste do Brasil). Vinte e cinco linhagens de vibrio foram avaliadas em PCR para integrons classe 1 em DNA cromossômico e plasmidial, utilizando os primers In, Int1, qacE∆1, sul1 e blaP1. Oito (32%) isolados produziram integrase (intI1) em DNA cromossômico pertencente a integrons classe 1, com o gene cassete blaP1 detectado em cepas de V. natriengens (V10), V. coralliilyticus (V11) e V. parahaemolyticus (V25). A porcentagem foi de 100% em plasmídeos, com a detecção dos cassetes gênicos blaP1 e ereA2 em V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) e V. mimicus (V22). A detecção de cassetes de genes de resistência antimicrobiana nas cepas isoladas indica a possibilidade de disseminação desses elementos genéticos para bactérias autóctones em ambiente de cultivo de camarão. |
| Abstract: | The purpose of the present study was to identify integrons and gene cassettes in vibrios isolated from the hemolymph of shrimp (Litopenaeus vannamei) farmed in Ceará (Northeastern Brazil). Twenty-five vibrio strains were evaluated on PCR for class 1 integrons in chromosomal and plasmidial DNA, using the primers In, Int1, qacE∆1, sul1 and blaP1. Eight (32%) isolates yielded integrase (intI1) in chromosomal DNA belonging to class 1 integrons, with the gene cassette blaP1 detected in strains of V. natriengens (V10), V. coralliilyticus (V11) and V. parahaemolyticus (V25). The percentage was 100% in plasmids, with the detection of the gene cassettes blaP1 and ereA2 in V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) and V. mimicus (V22). The detection of antimicrobial resistance gene cassettes in the isolated strains indicates the possibility of dissemination of these genetic elements to autochthonous bacteria in shrimp culture environment. |
| URI: | http://repositorio.ufc.br/handle/riufc/81250 |
| DOI: | 2236-6717 |
| Author's Lattes: | http://lattes.cnpq.br/0653219789872234 |
| Access Rights: | Acesso Aberto |
| Appears in Collections: | LABOMAR - Artigos publicados em revistas científicas |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2022_art_emrcarvalho.pdf | 789,72 kB | Adobe PDF | View/Open |
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