Avaliação do efeito do Dersani® e da água de coco liofilizada no modelo cutâneo de cicatrização por segunda intenção em ratos wistar

Abstract

Healing of wounds is a cascade of cellular and molecular events which interact with one another ensuing tissue reconstruction. This study assessed the effect of a compound medium-chain triglycerides, linoleic acid, soy lecithins and vitamins A and E (Dersan) and also lyophilized coconut water in the aqueous and gel forms in the process of healing of experimental cutaneous ulcers. 105 male Wistar rats were employed in which a 4cm2 full thickness skin segment was removed. The animals were randomly distributed into groups constituted of 15 rats comprising three experiments consisting of Control and Reference groups, as well as Amorphous-Aqueous and Crystalline-Aqueous groups (Experiment 1), Amorphous-gel and Crystalline-gel groups (Experiment 2) and Triglycerides (Experiment 3). Such groups were treated via topic, respectively with 0.9% saline, clostebol+neomycin sulphate, amorphous and crystalline coconut water, amorphous and crystalline-gel coconut water and Dersani. The wound areas were measured by digital planimetry at post-operative days zero, 3, 7 and 12; from them the degree and average rate of repair were calculated. Moreover, the healing process was assessed through the criteria of Meyers and the amount of collagen and angiogenesis by means of histochemical methods. At day 3, an expansion of the wound area in the Reference Group and a slight contraction of Control Groups and tests were noted. On the subsequent days the process of repair, measured through the variable degree of healing evolved in a linear form, so that on the twelfth day, the repaired area achieved 77.95% of the initial ulcerated region in the Control Group, 78.40% in the Reference Group, 82.24%, in the Amorphous-Aqueous one, 84.12% in the Crystalline- Aqueous (Experiment 1), 85.09% in the Amorphus-gel Group, 84.20% in the Crystalline-gel Group (Experiment 2) and 83.49% in that of Triglyceride (Experiment 3); no statistically significant differences were found in the experiments. Equally similar were the values for the average rate of repair in regard to the 12 days of treatment: 25,79mm2/day in the Control Group, 25,42mm2/day in the Reference Group, 26.48mm2/day in the Amorphous-Aqueous one, 27.89mm2/day in the Crystalline-Aqueous Group, 26,47mm2/day in the Amorphous-gel Group, 27.04mm2/day in the Crystalline-gel and 27.38mm2/day in the Triglyceride Group. However, as to the Groups Amorphous-aqueous (P<0,01), Amorphous-gel (P<0.05), Crystalline Gel (P<0,05) and Triglycerides (P<0,01), the density of collagen was significantly higher than that observed in the Control Group. Only the Group of Triglycerides showed significantly higher vascular density, deposition of collagen and the granulation tissue than Control and Reference groups (P<0, 01). These findings allow the conclusion that Dersani® have potential to the process of tissue repair