Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.ufc.br/handle/riufc/69565
Tipo: Artigo de Periódico
Título : Co-immobilization of dextransucrase and dextranase in epoxy-agarose- tailoring oligosaccharides synthesis
Autor : Silva, Rhonyele Maciel da
Souza, Priscila Maria Paiva
Fernandes, Fabiano André Narciso
Gonçalves, Luciana Rocha Barros
Rodrigues, Sueli
Palabras clave : Co-immobilization;Dextransucrase;Oligosaccharides;Epoxy-agarose;Eupergit CM
Fecha de publicación : 2019
Editorial : Process Biochemistry
Citación : FERNANDES, F. A. N. et al. Co-immobilization of dextransucrase and dextranase in epoxy-agarose- tailoring oligosaccharides synthesis. Process Biochemistry, [s.l.], v. 78, p. 71-81, 2019. DOI: https://doi.org/10.1016/j.procbio.2019.01.009
Abstract: The best support reported for dextransucrase (DS) and dextranase (DN) immobilization was Eupergit C, but this carrier was discontinued and is no longer available in the market. Thus, epoxy-agarose support is suggested as an alternative to co-immobilize DS from Leuconostoc mesenteroides B-512F and DN from Chaetomium erraticum. The co-immobilization approach improved DS performance, compared to immobilized DS, due to the removal of the dextran layer accumulated on DS by DN, and enhanced both enzymes catalytic activities under a wide range of pH and temperature. Since DS and DN have the same optimum pH and temperature, the co-immobilization was done in a single step. The EPA-DS-DN0.5 (co-immobilized DS (26.16 UI) and DN (2.76 UI) in epoxy-agarose) showed the highest recovered activity (59.54%). The biocatalyst was stable at 4 °C, retaining above 70% of activity for 60 days and was efficient producing oligosaccharides, yielding 12.68 ± 0.09 g/L of oligosaccharides with a degree of polymerization up to 5.
URI : http://www.repositorio.ufc.br/handle/riufc/69565
ISSN : 1359-5113
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