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Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, Rhonyele Maciel da | - |
dc.contributor.author | Souza, Priscila Maria Paiva | - |
dc.contributor.author | Fernandes, Fabiano André Narciso | - |
dc.contributor.author | Gonçalves, Luciana Rocha Barros | - |
dc.contributor.author | Rodrigues, Sueli | - |
dc.date.accessioned | 2022-11-25T17:39:23Z | - |
dc.date.available | 2022-11-25T17:39:23Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | FERNANDES, F. A. N. et al. Co-immobilization of dextransucrase and dextranase in epoxy-agarose- tailoring oligosaccharides synthesis. Process Biochemistry, [s.l.], v. 78, p. 71-81, 2019. DOI: https://doi.org/10.1016/j.procbio.2019.01.009 | pt_BR |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | http://www.repositorio.ufc.br/handle/riufc/69565 | - |
dc.description.abstract | The best support reported for dextransucrase (DS) and dextranase (DN) immobilization was Eupergit C, but this carrier was discontinued and is no longer available in the market. Thus, epoxy-agarose support is suggested as an alternative to co-immobilize DS from Leuconostoc mesenteroides B-512F and DN from Chaetomium erraticum. The co-immobilization approach improved DS performance, compared to immobilized DS, due to the removal of the dextran layer accumulated on DS by DN, and enhanced both enzymes catalytic activities under a wide range of pH and temperature. Since DS and DN have the same optimum pH and temperature, the co-immobilization was done in a single step. The EPA-DS-DN0.5 (co-immobilized DS (26.16 UI) and DN (2.76 UI) in epoxy-agarose) showed the highest recovered activity (59.54%). The biocatalyst was stable at 4 °C, retaining above 70% of activity for 60 days and was efficient producing oligosaccharides, yielding 12.68 ± 0.09 g/L of oligosaccharides with a degree of polymerization up to 5. | pt_BR |
dc.language.iso | en | pt_BR |
dc.publisher | Process Biochemistry | pt_BR |
dc.subject | Co-immobilization | pt_BR |
dc.subject | Dextransucrase | pt_BR |
dc.subject | Oligosaccharides | pt_BR |
dc.subject | Epoxy-agarose | pt_BR |
dc.subject | Eupergit CM | pt_BR |
dc.title | Co-immobilization of dextransucrase and dextranase in epoxy-agarose- tailoring oligosaccharides synthesis | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
Aparece nas coleções: | DEQ - Artigos publicados em revista científica |
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Arquivo | Descrição | Tamanho | Formato | |
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2019_art_fanfernandes.pdf | 3 MB | Adobe PDF | Visualizar/Abrir |
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