Contribuição ao conhecimento químico de espécies do gênero Hyptis do nordeste: H. martiusii Benth, H. platanifolia Mart ex. Benth, H. suaveolens (L.) Poit. e H. pectinata (L.) Poit.

Abstract

This work describes the chemical study of the volatile components of species of the genus Hyptis (Labiatae) endemic of the native flora of Northeastern Brazil- Hyptis platanifolia Mart ex. Benth (leaves and inflorescences), H.,vptis swaveolens (L) Poit. (leaves) and Hyptis pectinata (L.) Poit. (leaves), as well as the circadian study of the essential oil of Hyptis martrusil Benth (leaves and inflorescences). It was also accomplished the phytochemical study for isolation of the non volatile components of the species Hyptis martiusii Benth and Hyptis platanifolia Mart ex. Benth, and biological assays with the essential oil of the leaves, }IM-12 (1,8-cineol) and HM-1 (carnasol) and HM-2 (I 1,14-dihydroxy-8, I I,13-trien-7-one). The study of the essential oils resulted in the constituent identification of forty-six volatile constituents in the total, and the evaluation of the biological potential of the essential oil of the leaves of H. martiusii and 1,8-cineol, majority oxygenated monoterpene in the composition as insecticide agents. The chromatographic analysis of the extracts obtained for isolation of non volatile components resulted in the further characterization of three abietane diterpenes, a triterpene and a steroids mixture, for H. martiusii Benth in the characterization of five abietane diterpenes, two triterpenes and a steroids mixture for H platanifolia Mart ex. Benth. Of the isolated substances three are unpublished in the literature and all isolated substances are unpublished for the species. The study of the antiproliferative effect of H1M-1 and HM-2, isolated of IL martiusii Benth, revealed that the tested substances possess low toxidade the cell. The identification of the volatile constituents was accomplished by GC-MS after Kovat's indices and mass spectra comparison with data from a computer library, as well visual comparison to published spectral data. Spectrometric analysis such as infrared and nuclear magnetic ressonance including special pulse sequences such as 111, COSY and 'H_ '3C-COSY n=1, 2 e 3) with normal and inverse detection, and comparison to literature data was utilized to structural determination of the non-volatile constituents and chemical derivatives.