Mostrando tradução para Enzymatic hydrolysis of the raw muscle of nurse Shark, Ginglymostoma cirratum Em vez disso, traduzir Enzimatic hydrolysis of the raw muscle of nurse Shark, Gynglimostoma cirratum 77/5000 Hidrólise enzimática do músculo cru do enfermeiro Shark, Ginglymostoma cirratum

Abstract

This work describes the process of preparing the hydrolyzate of the raw muscle of sandpaper, Gynglimostoma cirratum. The muscle was filleted and the meat was washed five times with distilled water, following homogenization with water in the proportion of 1: 2 (w / v) and adjusting the pH of the mixture to 7.5 and then pancreatin, propaine or protease was added in concentrations of 0.1%, 0.2%, 0.3%, 0.4% and 0.5% in relation to the substrate. Hydrolysis was carried out at 40 ° C in a water bath with mechanical agitation during the times of 5, 10, 1, 30, 60, 90 and 120 min. The rate of the enzymatic reaction was measured by the degree of hydrolysis according to the method described by Adler-Nissen & Olsen (1979). The statistical results showed that there is a significant difference between the enzyme treatments, pointing out the best results for pancreatin at 0.4% and 0.5%, and the time between 90 min. and 120 min. Taking into account the expenses with the enzyme and the exposure of the product to a long period and the temperature favorable to bacterial growth, it is suggested as the best result the concentration of 0.4% and the time of 90 min.