Pré-tratamentos térmicos e meios de cultivos na calogênese de anteras de meloeiro

Abstract

Melon is a plant of great economic importance. Haploidization is the process of producing haploid plants in a single generation.After this step, a dihaploidization will occir to obtain pure homozygotes. This technique has been applied in many cultures. Although it is not improved, with na efficiency of this method that was low. An alternative for the production of melon-tree haploid genotypes is by in vitro anther culture. Cantalupensis and Inodorus were the botanical varieties used. The explantes captured were the excited anthers of male flowers in the pre-anthesis stage of plants keept in a greenhouse. The selected materials were applied in two stages,in the callus induction stage, as the anthers were placede in basic cultire medium. The experimental design was randomized in two botanical varieties, three pre-applied, being the control, heat and cold, remainig in the growt room for 28 days, obtaining a callus formation. The calculations were transferred to three different new maturation mehotds. Two weeks after the start of this step, the difference occurs betwenn each of the previous factors: color, size and scores. Sixty-three days after inoculation in the first step, if an evoluation in material, assigned to a grade where no variety of cantalupensis can be observed the best results.