Utilização do peptídeo sintético aureína k como adjuvante na atividade antimicrobiana de ciprofloxacina contra pseudomonas aeruginosa atcc 9027

Abstract

Much of the microorganisms are in nature organized in the form of biofilm, which provides greater protection and resilience through factors adverse. Pseudomonas aeruginosa is an animal and human pathogen that is often associated with nosocomial infections that are difficult to eradicate and which They are extremely easy to form biofilms. Antibiotics like ciprofloxacin are considered of first choice in the treatments against this microorganism, in the However, resistance mechanisms have been developed by this bacterium. Of this Thus, it is necessary to study new forms of control that can replace or potentiate conventional methods. To this end, antimicrobial peptides (PAMs), extracted from various living organisms, may represent an alternative for the development of new drugs. Aurein family peptides as well as like its analogous forms, has been presenting good results among which it can be highlight aurein 1.2 (AU) and aurein K (K-au). Thus, the objective of this paper was to evaluate the influence of synthetic aurein K (K-au) peptide on the activity ciprofloxacin antimicrobial activity against P. aeruginosa ATCC 9027. The influence of Kau IPC antimicrobial activity was verified by the checkerboard methodology quantification of biomass and the number of viable cells in the biofilm by violet crystal staining and colony forming units (CFU) count, respectively. The K-au MIC and CBM values ​​were 31.25 μg.mL-1 and 62, 25 μg.mL-1, and for CIP was 0.24 and 0.48 μg.mL-1, respectively. The data showed whereas both CIP and K-au are potent antimicrobial agents against P. aeruginosa, being capable of inhibiting planktonic growth and reducing preformed P. aeruginosa biofilms. The interaction of substances was interpreted by the fractional inhibitory concentration index (ICIF) and showed a a 4-fold decrease in CIM and 2 in CIP combined with K-au. This influence was also observed both in the kinetics of death, antibiotic activity, and cellular metabolism. In conclusion, the effect of the CIP / K-au combination suggests that the peptide has potential as an agent and adjuvant treatment for the treatment of infectious diseases caused by P. aeruginosa, possibly enabling the use of lower concentrations than antibiotic.