Fracionamento do extrato de casca de manga (Mangifera indica L.) CV. Tommy Atkinns para obtenção de mangiferina por HPLC-UV/VIS preparativo

Abstract

Mango (Mangifera indica L.) belonging to the Anarcadiaceae family is one of the most cultivated fruits in the world. Agroindustrial activity generates high amounts of waste that be used for recovery of bioactive compounds with important applications for the pharmaceutical, cosmetic and food industries, for example, becoming an alternative to revert the environmental and economic impacts from waste generation. Therefore, the objective of this work is the fractionation of mango bark extract to obtain phenolic compounds such as mangiferin (xanthone) and other metabolites. The methanolic extract of mango peel obtained in the Laboratório Multiusuário de Química de Produtos Naturais located at Embrapa Agroindústria Tropical by Soxleht extraction and concentrated in a rotary evaporator under reduced pressure, and then lyophilized. The chromatographic separation of the analytical HPLC-DAD extract was conducted in the exploratory mode using a Shimadzu Shim-pack CLC-ODS (M) C18 (250 mm x 4.6 mm) phase-reversed chromatography column. The mobile phase used was composed of methanol and water, both acidified with 0.05% (v/v) trifluoroacetic acid, in an elution gradient with 60 minutes chromatographic analysis. Fractionation was performed on a preparative scale using a Waters SunFire Prep C18 OBD reverse phase chromatographic column (100 mm x 19 mm) using the same analytical scale method with a 45 minutes chromatographic analysis. In both analyzes the chromatograms were monitored at a wavelength of 280 nm. The structural confirmation of mangiferin and other secondary metabolites performed by UPLC-QTOF-MSE using the molecular formulas and m/z values obtained from high-resolution spectra, observed in the chromatograms with higher intensity peaks, by means of Mass Lynx software (Water Corporation).