Profundidade de penetração de dois cimentos obturadores endodônticos em função do fluoróforo e da umidade dentinária: estudo em microscopia confocal

Abstract

The objective of the present study was to determine the depth of penetration in the dentin tubules provided by two endodontic obturator cements as a function of the moisture present in the root canal system and the fluorophore used. For this, root canals of the 40 upper molar distobuccal roots were prepared (Reciproc R25) and filled with gutta-percha cones associated with AH Plus (AH) and Endofill (EF) cements incorporated with two fluorophores, Rhodamine- B ) and Fluo-3 (F3); they were mixed simultaneously to the cements at the time of manipulation (0.1g/g). The specimens were randomly divided into 4 groups (n = 10) as a function of cement (AH / EF) and the condition of the dentin substrate (wet / dry). The roots that maintained moist substrate were dried only with brief aspiration followed by cones of absorbent paper; for moisture removal the roots were kept in an oven at 70ºC for 7 days. Seven days after obturation the specimens were sectioned transversely at 2, 4 and 6 mm of the anatomic apex; the sections were prepared and taken to the Confocal Laser Scanning Microscope to determine the depth of penetration of the dentin tubules (mm) in the Image J. software. The multifactor analysis showed superiority for the AH Plus cement, which presented greater penetration in the (P < 0.05), however, no significant differences were observed regarding the fluorophore used (ROD / F3) or the dentin moisture condition (P > 0.05). In relation to the thirds, statistically significant differences were observed, with the apical third presenting less penetration when compared to the others (P < 0.05). Under the conditions of the study, it was possible to conclude that the AH Plus cement presented higher intratubular penetration than the Endofill cement, independent of the other variables tested - moisture or fluorophore - that did not interfere with penetration; the highest penetrations were observed in the cervical and middle thirds.