Tempo de permanência in vitro, na fase de alongamento e enraizamento, na aclimatização de mudas micropropagadas de abacaxizeiro ornamental

Abstract

In order to reduce the costs of production process of micropropagated seedlings of ornamental pineapple, this study aimed to develop one protocol, fitting the best time of permanence of shoots in the elongation phase and rooting in vitro, for acclimatization of seedlings. The experiment was accomplished with Laboratorio de Cultura de Tecidos Vegetais placed on Embrapa Agroindustria Tropical, using hybrid shoots A. comosus var. bracteatus x A. comosus var. erectifolius – PL 16(called access D), elongated and rooted in the middle of culture MS added 0,1mgL-1of naftalenoacetico acid (ANA) and acclimatized on trays of 162 cell in substrate HS Florestal for 60 days in a greenhouse. The study defined 5 treatments T1( 28 days), T2 (35 days), T3 (42 days), T4 (49 days), T5 (56 days), analyzed in accord with the stay time of culturas in vitro during elongation and rooting. The shoots which were kept in elongation and rooting phase in vitro for 35 days showed better development for height, number of leaves, colmo diameter, lenght of bigger root, fresh pasta of aérea and root part, during the acclimatization. The investigation concludes that the elongated and rooted shoots in 35 days only showed satisfastory performance in acclimatization.