Desreplicação do extrato aquoso de Phyllanthus Niruri Linn por ressonância magnética nuclear (Rmn)

Abstract

Phyllanthus niruri (Euphorbiaceae) is a medicinal plant that is popularly recognized for its diuretic properties. This plant is being used in removing kidney stones. In this work, it was the chromatography profile was evaluated and the use of a rapid and efficient methodology to identify and characterize the chemical constituents. The extract of the leaves and fruits of P. niruri was obtained through accelerated solvent extraction (ASE) using water as a solvent extraction and, subsequently subjected to reversed-phase liquid chromatography. Ten fractions were obtained and analyzed by Liquid Chromatography coupled to mass spectrometry (UPLCQTOF) and Nuclear Magnetic Resonance (NMR). The chemical investigation was done with the aqueous extract from the fruits and leaves of the referred species, which resulted in the characterization of nine substances: repandusinic acid A (39), kaempferol-O-galloylrhamnoside (55), corilagin (41), phyllanthusiin C (42), phyllanthusiin U(43), furosin (44), geraniin (45), rhoipteleanins H (54) and vitexin-2’’-O-rhamnoside (25). The structures of all isolated substances were elucidated by spectrometric methods (MS-IES e NMR 1H e 13C 1D e 2D) and comparison with literature data.