Potencial antidepressivo do timol: Efeitos sobre os níveis do fator neurotrófico derivado do cérebro (BDNF) no modelo de estresse crônico induzido por corticosterona em camundongos

Abstract

Major Depressive Disorder (MDD), better known as depression, is a pathology that attracts thousands of people, being the force of functional disability and impairment of physical and mental health. There are many theories that explain the depressive picture, but currently the most accepted would be the monoaminergic theory of depression. Linked to this theory would be the neurogenesis, responsible for neuronal plasticity and known as expressed by a non-depressive behavioral pattern. Based on this and the enhanced for pharmaceuticals of this type, the central therapeutic effects of Thymol, a terpene with several therapeutic actions, including the antidepressant activity derived from Lippia sidoides, were verified in the neurogenic pathway, seeking to elucidate the involvement of the molecule and depression . To perform the study, chronic subcutaneous administration of corticosterone was used in swiss females to induce depressive symptoms for 23 days. From the 14th day of corticosterone, Timol, a standard positive drug (Fluvoxamine) and vehicle was administered to be made comparative. At the end of the 23-day treatment of the behavioral tests were initiated and, at the end of all, the animals sacrificed by guillotine were dissected for hippocampal evaluation of BDNF. Test objects were performed, forced swim test, tail suspension, sucrose preference, light / dark, social interaction, y-maze, plus-maze, hole-board, to access memory, depression and anhedonia. To assess the level of efficacy, ELISA and Western Blot, to assess BDNF levels, this factor is involved in cellular neurogenesis. As a result, Thymol and Fluvoxamine were able to reverse the depressive symptoms assessed by forced-swim and tail-suspension tests, as well as to improve the anxious frame measured by hole-board, plus-maze and social interaction. The anhedonic and idle memory in the memory of problems was restored with the treatment. In the neurochemical tests both Thymol and Fluvoxamine restored BDNF levels, improving the depressive condition. This work aimed to evalueted the interaction of Thymol with the neurogenic pathway-BDNF, opening up precedents for new investigations aiming the use of the molecule as a therapeutic alternative for this pathology.