Proteínas isoladas do látex de Himatanthus drasticus (MART.) Plumel apocynaceae reduzem a resposta inflamatória e nociceptiva na artrite induzida por zymosan em camundongos

Abstract

Himatanthus drasticus is a laticiferous plant of the family Apocynaceae found in South America and popularly known as janaguba. Your latex is known as "janaguba milk" and has several pharmacological activities. This work aimed to study the effect of isolated proteins from Himatanthus drasticus latex (HdLP) zymosan (Zy) induced arthritis. Arthritis was induced in male Swiss mice (n = 6) by administering 0.1 mg Zy intra-articular. The animals were treated with HdLP (5 and 50 mg/kg i.v.) or Saline i.v. 1 hour after the Zy and HdLP (50 mg/kg v.o.) or Dexamethasone (2 mg/kg s.c.) 1 hour before Zy. After six hours of induction the animals were euthanized, the joint washing carried, and collected for leukocyte count, determination of the myeloperoxidase (MPO) activity and inflammatory mediators (NO, TNF-α, IL-1β, IL-6 and IL-10). The edema was evaluated by measuring the diameter articular and the vascular permeabilitym through extravasation. In addition, we evaluated the effect of HdLP on synovial inflammation as well the mechanical hypernociception. For statistical analysis we used the ANOVA/Bonferroni or Kruskal Wallis/Dunns; p <0.05 considered significant. This study was approved by the Ethics Committee on Animal Research of the UFC - CEPA (38/14). HdLP (50 mg/kg i.v.) a significant decrease on the leukocyte count in MPO activity and levels (NO, IL-1β, and IL-6). It was also observed a decrease in swelling and damage to the synovial membrane. The nociceptive threshold was increased by HdLP 50 mg/kg i.v. and 50 mg/kg v.o. HdLP has antinociceptive and anti-inflammatory activity in the model of arthritis induced by Zy. The effect seems to be related to the decreased migration of neutrophils and the release of inflammatory mediators, reducing damage to the synovial membrane and hypernociception.