Avaliação da toxicidade aguda e subcrônica da torta e farelo de mamona destoxificados em ratos

Abstract

This study aimed to evaluate the acute and subchronic toxicity of the by-products of Ricinus communis (castor oil plant) detoxified by CaO and the desolventized castor oil plant bran in female and male Wistar rats, in order to verify their viability as animal feed. The detoxified castor bran and castor cake were supplied by EMBRAPA. The processing of these byproducts was performed in order to extract the soluble proteins to perform the treatment. Wistar rats (150g), male and female, were used. Acute toxicity test (2,000 mg/kg) was performed in females, according to OECD 423 protocol. Subchronic tests were performed in males and females divided in control (NaCl 0.9%) and subchronic groups (5, 50 and 300 mg/kg of castor meal and castor cake), according to OECD 407. Weight, hypocratic screening, AST, ALT, Urea, Creatinine of all animals and complete blood count of males were evaluated. The results showed that the survival rate was 94.56 %, with gradual weight gain, without significant differences between the groups. No significant changes were observed in the evaluation of the hypocratic screening. Regarding biochemical and hematological evaluations, it was observed that AST levels in females treated with the bran increased in the acute group (P<0.0172) compared to the control group and with the subchronic treatments: 5 mg/kg (P<0.0001), 50 mg/kg (P<0.0006) and 300 mg/kg (P<0.0001). In females treated with the pie, AST increased in the acute group when compared to the control group (P<0.0002) and also in relation to the subchronic, 5 mg/kg (P<0.0001), 50 mg/kg (P<0.0001) and 300 mg/kg (P<0.0001), similar result was obtained with ALT in the acute group (63.3 ± 10.4), which increased when compared to the control group (P< 0.0016). Urea in females treated with the acute bran (2000 mg/kg) showed an increase when compared to the control group (P< 0.0001) and with the subchronic treatments. No significant changes were observed in hematological parameters in the pie or bran treated groups when compared to the control group. The changes observed in the treated animals, although significant compared to the control animals, did not exceed the reference levels, thus not demonstrating toxicity of the detoxified cake and bran compounds, at the doses and acute and subchronic treatments tested