Atividade antibiofilme isolada e combinada de peptídeos sintéticos e antifúngicos contra Candida albicans e C. krusei

Abstract

The Candida genus is widely studied was recently recognized by the World Health Organization as a high-risk pathogen for humans. Due to the rising problem of microbial resistance, which is generally caused by drug misuse and the development of defense mechanisms in the microbial cells, a search for new treatment alternatives for diseases caused by these microorganisms. In the present issue, the antibiofilm potential of 4 synthetic antimicrobial peptides was tested, both alone and combined with the commercial drugs Nystatin and Itraconazole. The essays were conducted in 96 well polystyrene plates in which Candida albicans and Candida krusei were inoculated with the treatments and incubated for 48 hours to verify the inhibition of biofilm formation and the reduction of biofilm biomass. After the incubation phase, the biofilm was isolated, dyed and quantified using a microplate reader at 590nm. In this issue, was found a high activity (60% to 80%) both in inhibition of biofilm formation and reduction of biofilm biomass alongside all peptides. The combination with itraconazole showed an non interaction or minor increase in activity while the combination with Nystatin showed an antagonist effect, in witch was observed a loss in activity when combining the peptides with this antifungal.