Efeito do extrato de própolis hidroalcoólico como agente biomodificador de colágeno dentinário na preservação da interface adesiva

Abstract

The adhesive/dentin interface is the most fragile link for adhesive systems, as the collagen fibers exposed from acid etching are not fully infiltrated by the monomers of the adhesive systems, leading to the action of degradative mechanisms. Currently, there are several strategies to reduce this damage, such as the use of dentin collagen biomodifying agents. Propolis extract is a resinous substance, collected by bees and which has several polyphenols in its chemical structure, so it can interact with dentin collagen. The objective of the study was to evaluate the effect of Hydroalcoholic Propolis Extract (EPH) as a dentin collagen biomodifying agent in preserving the adhesive interface. To this end, the research consists of an in vitro laboratory study, in which healthy human third molars were used to make dentin bars (1.7 X 0.5 X 6 mm), which were demineralized with phosphoric acid at 10 % for 5 hours and distributed into the following groups: (1) Distilled water (CT); (2) Grape Seed Extract (ESU) 6.5% (positive control); (3) EPH 0.1%; (4) EPH 1% and (5) EPH 10%. The samples obtained were quantitatively evaluated using the Elasticity Modulus [ME] (n=10) and Mass Measurement [MM] (n=10); Fourier Transform Infrared Spectroscopy [FT-IR] (n=10) was also performed for qualitative assessment of the bonds formed and Bond Strength [RU] (n=8). The data were subjected to statistical analysis using the SigmaStat program, followed by the Kolmogorov-Smirnov normality test and two-way analysis of variance (ANOVA) for RU and repeated measures for ME and MM (p<0.05). As for ME, the ESU 6.5% group demonstrated gains in the immediate period (9.75 ± 3.315) and the results of the test groups EPH 0.1% (3.83 ± 2.33) and EPH 1% (4 .53 ± 2.71) were also significant in the immediate assessment. In MM, the EPH 1% group was the only one to promote mass gain over a period of 7 days (2.84 ± 5.48). In RU, the test groups were able to maintain RU after 6 months of storage in distilled water, except for the 0.1% EPH group (31.7 ± 0.03). Regarding FT-IR, all groups showed an increase in amide I peaks, but there was a reduction in amide II peaks, which are associated with the formation of collagen cross-links after the cross-linking process. It is concluded that EPH can be a promising agent in Restorative Dentistry, especially at a concentration of 1%, which showed positive effects in all tests carried out.