Isolamento, domesticação e cultivo de microalgas de ambientes lacustres impactados de Fortaleza-CE e região metropolitana, para uso em aquicultura

Abstract

Microalgae have been the subject of several studies by having a great versatility of use, whether in industry to produce biodiesel, pharmaceuticals, environmental as bioindicator as well as for food and feed. This study deals with the isolation, domestication and cultivation of microalgae derived from the lagoons of Maraponga, Messejana, Mondubim, Parangaba and Opaia in Fortaleza and Tabapuã one lagoon in Metropolitan region. The samples were collected on May 21, 2013. The experiment was conducted in Planctologia Laboratory located in Applied Biotechnology Center for Aquaculture (CEBIAQUA), Department of Fisheries Engineering, Federal University of Ceará (UFC). With the completion of the work, was performed isolation and identification of seven taxons belonging to classes Chlorophyceae, Trebouxiophyceae, Cyanophyceae e Bacillariophyceae, being four identified to species level and three at genus. The stationary cultures or "baths" were performed using medium Guillard f / 2 at temperature of 24 ± 2 ° C, with lighting and aeration continues, with population growth of cultures accompanied by spectrophotometry by optical density of the medium (OD 680 and 700 nm). The biomass yield (g / L) of each culture was determined in the exponential phase being obtained by total suspended solids analysis. With regard to biomass yield, ranged from 0.35 to 0.327 g L-1. Isolation in Guillard f / 2 demonstrated efficacy to obtain the isolated strains. Producing microalgal biomass isolated were possible.