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Tipo: Artigo de Periódico
Título : A chromophore-containing agglutinin from Haliclona manglaris: Purification and biochemical characterization
Autor : Carneiro, Rômulo Farias
Almeida, Alexandra Sampaio de
Melo, Arthur Alves de
Alencar, Daniel Barroso de
Sousa, Oscarina Viana de
Delatorre, Plínio
Nascimento, Kyria Santiago do
Saker-Sampaio, Silvana
Cavada, Benildo Sousa
Nagano, Celso Shiniti
Sampaio, Alexandre Holanda
Palabras clave : Lectin;Chromophore;Sponge;Lecitina;Cromóforo;Esponja
Fecha de publicación : 2015
Editorial : International Journal of Biological Macromolecules
Citación : CARNEIRO, Rômulo Farias; ALMEIDA, Alexandra Sampaio de; MELO, Arthur Alves de; ALENCAR, Daniel Barroso de; SOUSA, Oscarina Viana de; DELATORRE, Plínio; NASCIMENTO, Kyria Santiago do; SAKER-SAMPAIO, Silvana ; CAVADA, Benildo Sousa; NAGANO, Celso Shiniti; SAMPAIO Alexandre Holanda. A chromophore-containing agglutinin from Haliclona manglaris: Purification and biochemical characterization.International Journal of Biological Macromolecules, Netherlands, v. 72, p. 1368-1375, 2015. Disponível em: https://doi.org/10.1016/j.ijbiomac.2014.10.003. Acesso em: 08 ago. 2023.
Abstract: A new chromophore-containing agglutinin (Haliclona manglaris agglutinin (HMA)) was isolated from the tropical sponge H. manglaris. HMA was purified by a combination of hydrophobic interaction chromatography and ion exchange chromatography. Native HMA is a heterotrimer formed by two β-chains (15 kDa) and one α-chain (22 kDa). HMA is a glycoprotein and possesses three intrachain disulfide bonds. Hemagglutinating activity of HMA was stable at neutral pH and temperatures up to 60 °C. HMA was only inhibited by thyroglobulin. Mass spectrometry sequencing and Edman degradation revealed a unique amino acid sequence of about 30%. Moreover, HMA has an organic chromophore of 581 Da, and this characteristic seems to be important to its antioxidant activity. Interestingly, while HMA showed no toxicity against Artemia nauplii and was unable to agglutinate bacterial cells, it did show a high capacity to protect β-carotene against oxidation. Thus, our findings suggest the putative involvement of HMA in the protection of the sponge against oxidation.
URI : http://www.repositorio.ufc.br/handle/riufc/73886
ISSN : 1879-0003
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