Redox mediator, microaeration, and nitrate addition as engineering approaches to enhance the biotransformation of antibiotics in anaerobic reactors

Abstract

The present work assessed some engineering approaches, such as the addition of the redox mediator anthraquinone-2,6-disulfonate (AQDS) (50 and 100 μM), microaeration (1 mL air min−1), and nitrate (100−400 mg L−1), for enhancing the biotransformation of the antibiotics sulfamethoxazole (SMX) and trimethoprim (TMP) (200 μg L−1 each) in anaerobic reactors operated at a short hydraulic retention time (7.4 h). Initially, very low removal efficiencies (REs) of SMX and TMP were obtained under anaerobic conditions (∼6%). After adding AQDS, the anaerobic biotransformation of these antibiotics significantly improved, with an increase of approximately 70% in the REs with 100 μM of AQDS. Microaeration also enhanced the biotransformation of SMX and TMP, especially when associated with AQDS, which provided REs above 70%, particularly for TMP (∼91% with 1 mL air min−1 and 50 μM of AQDS). Concerning nitrate, the higher the added concentration, the higher the REs of the antibiotics (∼86% with 400 mg L−1). Therefore, all the assessed approaches were demonstrated to be very effective in improving the limited biotransformation of SMX and TMP in anaerobic reactors, ensuring REs comparable to those found in higher-cost wastewater treatment technologies, such as conventional activated sludge, membrane bioreactors, and hybrid processes.