Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.ufc.br/handle/riufc/67709
Tipo: Artigo de Periódico
Título : RNAi-based inhibition of infectious myonecrosis virus replication in Pacific white shrimp Litopenaeus vannamei
Autor : Feijó, Rubens Galdino
Maggioni, Rodrigo
Martins, Pedro Carlos Cunha
Abreu, Keuly Ladislau de
Oliveira Neto, João Mafaldo
Guertler, Cristhiane
Justino, Emily Bruna
Perazzolo, Luciane Maria
Marins, Luis Fernando
Palabras clave : Specie - Litopenaeus vannamei;Rna interference (RNAi);Gene expression;Espécie - Litopenaeus vannamei;Expressão gênica
Fecha de publicación : 2015
Editorial : Diseases of Aquatic Organisms
Citación : FEIJÓ, Rubens Galdino; MAGGIONI, Rodrigo; MARTINS, Pedro Carlos Cunha; ABREU, Keuly Ladislau de; OLIVEIRA NETO, João Mafaldo; GUERTLER, Cristhiane; JUSTINO, Emily Bruna; PERAZZOLO, Luciane Maria; MARINS, Luis Fernando. RNAi-based inhibition of infectious myonecrosis virus replication in Pacific white shrimp Litopenaeus vannamei. Diseases of Aquatic Organisms, Germany, v. 114, p. 89-98, 2015.
Abstract: Disease in Pacific white shrimp Litopenaeus vannamei caused by the infectious myonecrosis virus (IMNV) causes significant socioeconomic impacts in infection-prone shrimp aquaculture regions. The use of synthetic dsRNA to activate an RNA interference (RNAi) response is being explored as a means of disease prophylaxis in farmed shrimp. Here, survival was tracked in L. vannamei injected with long synthetic dsRNAs targeted to IMNV open reading frame (ORF) 1a, ORF1b, and ORF2 genome regions prior to injection challenge with IMNV, and real-time RTPCR was used to track the progress of IMNV infection and mRNA expression levels of the host genes sid1, dicer2, and argonaute2. Injection of dsRNAs targeting the ORF1a and ORF1b genes but not the ORF2 gene strongly inhibited IMNV replication over a 3 wk period following IMNV challenge, and resulted in 90 and 83% shrimp survival, respectively. Host gene mRNA expression data indicated that the Sid1 protein, which forms a transmembrane channel involved in cellular import/export of dsRNA, increased in abundance most significantly in shrimp groups that were most highly protected by virus-specific dsRNA injection. Subclinical IMNV infections present in the experimental L. vannamei used increased markedly in the 2 d between injection of any of the 4 virus-specific or non-specific dsRNAs tested and IMNV challenge. While handling and injection stress are implicated in increasing IMNV replication levels, the underlying molecular factors that may have been involved remain to be elucidated.
URI : http://www.repositorio.ufc.br/handle/riufc/67709
ISSN : 0177-5103
Aparece en las colecciones: LABOMAR - Artigos publicados em revistas científicas

Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
2015_art_rgfeijo.pdf1,43 MBAdobe PDFVisualizar/Abrir


Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.