Please use this identifier to cite or link to this item: http://repositorio.ufc.br/handle/riufc/67046
Type: Artigo de Periódico
Title: Genetic manipulation of native Bacillus cereus : a biotechnological tool for aquaculture
Authors: Costa Filho, João
Riet, Jade Oliveira
Santos, Kamila
Sousa, Oscarina Viana de
Maggioni, Rodrigo
Feijó, Rubens Galdino
Wasielesky Júnior, Wilson Francisco Brito
Marins, Luis Fernando
Keywords: Biotechnology;Probitoic;Speciation - Litopenaeus vannamei;Biotecnologia;Probiotico;Especiação - Litopenaeus vannamei
Issue Date: 2020
Publisher: Journal of Applied Aquaculture
Citation: COSTA FILHO, João; RIET, Jade ; SANTOS, Kamila ; SOUSA, Oscarina Viana de; MAGGIONI, Rodrigo ; FEIJÓ, Rubens Galdino; WASIELESKY Jr, Wilson; MARINS, Luis Fernando. Genetic manipulation of native Bacillus cereus : a biotechnological tool for aquaculture. Journal of Applied Aquaculture, United States, v. 32, p. 1-11, 2020.
Abstract: This work aimed to perform the genetic manipulation of the native B. cereus #25 strain and to evaluate the colonization in the intestinal tract of Litopenaeus vannamei. The expression of the GFPmut1, absence of amylase activity, and growth in spectinomycin plates confirmed the transformation of the strain (BC25GM). In the shrimp bioassay, its addiction in the feed showed non-difference for water quality, growth performance, and toxic effects for L. vannamei (survival 100%). The presence of cells expressing GFPmut1 in the midgut of the animals was observed, suggesting that the genetically engineered strain colonized the shrimp intestines. The relative fluorescence was 35% higher in the midgut of the shrimps feed with BC25GM. In conclusion, the present work showed that it is possible to manipulate a native B. cereus strain with the nonspecific pSG1154 plasmid. We reported the possibility to implement native strains as a biotechnology tool applied in aquaculture, especially to deliver beneficial molecules for shrimps.
URI: http://www.repositorio.ufc.br/handle/riufc/67046
ISSN: 1545-0805
Appears in Collections:LABOMAR - Artigos publicados em revistas científicas

Files in This Item:
File Description SizeFormat 
2020_art_jcostafilho.pdf2,52 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.