Please use this identifier to cite or link to this item: http://repositorio.ufc.br/handle/riufc/66484
Type: Artigo de Periódico
Title: Dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in an experimental model of acute kidney injury
Authors: Freitas, Flavia Priscila Santos
Porto, Marcella Leite
Tranhago, Camilla da Penha
Piontkowski, Rogerio
Miguel, Emilio de Castro
Miguel, Thaiz Batista Azevedo Rangel
Martins, Jorge Luiz
Nascimento, Kyria Santiago do
Balarini, Camille de Moura
Cavada, Benildo Sousa
Meyrelles, Silvana dos Santos
Vasquez, Elisardo Corral
Gava, Agata Lages
Keywords: Acute kidney injury;Ischemia reperfusion;Renal function;Plant lectin;Dioclea violacea
Issue Date: 2015
Publisher: American Journal of Translational Research
Citation: FREITAS, Flavia P. S. et al. Dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in an experimental model of acute kidney injury. American Journal of Translational Research, [s. l.], v. 7, n. 12, p. 2573–2588, 2015.
Abstract: Acute kidney injury (AKI) is characterized by rapid and potentially reversible decline in renal function; however, the current management for AKI is nonspecific and associated with limited supportive care. Considering the need for more novel therapeutic approaches, we believe that lectins from Dioclea violacea (Dvl), based on their antiinflammatory properties, could be beneficial for the treatment of AKI induced by renal ischemia/reperfusion (IR). Dvl (1 mg/kg, i.v.) or vehicle (100 µL) was administered to Wistar rats prior to the induction of bilateral renal ischemia (45 min). Following 24 hours of reperfusion, inulin and para-aminohippurate (PAH) clearances were performed to determine glomerular filtration rate (GFR), renal plasma flow (RPF), renal blood flow (RBF) and renal vascular resistance (RVR). Renal inflammation was assessed using yeloperoxidase (MPO) activity. Kidney sections were stained with hematoxylin-eosin to evaluate morphological changes. Intracellular superoxide anions, hydrogen peroxide, peroxynitrite, nitric oxide and apoptosis were analyzed using flow cytometry. IR resulted in diminished GFR, RPF, RBF, and increased RVR; however, these changes were ameliorated in rats receiving Dvl. AKI-induced histomorphological changes, such as tubular dilation, tubular necrosis and proteinaceous casts, were attenuated by Dvl administration. Treatment with Dvl resulted in diminished renal MPO activity, oxidative stress and apoptosis in rats submitted to IR. Our data reveal that Dvl has a protective effect in the kidney, improving renal function after IR injury, probably by reducing neutrophil recruitment and oxidative stress. These results indicate that Dvl can be considered a new therapeutic approach for AKI-induced kidney injury.
URI: http://www.repositorio.ufc.br/handle/riufc/66484
ISSN: 1943-8141
Appears in Collections:DEMM - Artigos publicados em revista científica

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