Please use this identifier to cite or link to this item: http://repositorio.ufc.br/handle/riufc/59841
Type: Artigo de Periódico
Title: Crotacetin, a Novel Snake Venom C-Type Lectin Homolog of Convulxin, Exhibits an Unpredictable Antimicrobial Activity
Title in English: Crotacetin, a Novel Snake Venom C-Type Lectin Homolog of Convulxin, Exhibits an Unpredictable Antimicrobial Activity
Authors: Rádis-Baptista, Gandhi
Moreno, Frederico Bruno Mendes Batista
Nogueira, Lucas de Lima
Martins, Alice M. C.
Toyama, Daniela de Oliveira
Toyama, Marcos Hikari
Cavada, Benildo Sousa
Azevedo Júnior, Walter Filgueira de
Yamane, Tetsuo
Keywords: Toxina;Veneno;Cobras
Issue Date: 2006
Publisher: Cell Biochemistry and Biophysics
Citation: RÁDIS-BAPTISTA, Gandhi; MORENO, Frederico Bruno Mendes Batista; NOGUEIRA, Lucas de Lima; MARTINS, Alice M. C.; TOYAMA, Daniela de Oliveira; TOYAMA, Marcos Hikari; CAVADA, Benildo Sousa; AZEVEDO JÚNIOR, Walter Filgueira de; YAMANE, Tetsuo. Crotacetin, a Novel Snake Venom C-Type Lectin Homolog of Convulxin, Exhibits an Unpredictable Antimicrobial Activity. Cell Biochemistry and Biophysics, United States, v. 44. 2006.
Abstract: Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins that are capable of interfering with blood stasis. A very well-studied svC-type lectin is the heterodimeric toxin, convulxin (CVX), from the venom of South American rattlesnake Crotalus durissus terrificus. CVX is able to activate platelets and induce their aggregation by acting via p62/GPVI collagen receptor. By using polymerase chain reaction homology screening, we have cloned several cDNA precursors of CVX subunit homologs. One of them, named crotacetin (CTC) β-subunit, predicts a polypeptide with a topology very similar to the tridimensional conformations of other subunits of CVXlike snake toxins, as determined by computational analysis. Using gel permeation and reverse-phase high-performance liquid chromatography, CTC was purified from C. durissus venoms. CTC can be isolated fromthe venom of several C. durissus subspecies, but its quantitative predominance is in the venom of C. durissus cascavella. Functional analysis indicates that CTC induces platelet aggregation, and, importantly, exhibits an antimicrobial activity against Gram-positive and -negative bacteria, comparable with CVX.
URI: http://www.repositorio.ufc.br/handle/riufc/59841
ISSN: 1085-9195
Appears in Collections:LABOMAR - Artigos publicados em revistas científicas

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