Please use this identifier to cite or link to this item: http://repositorio.ufc.br/handle/riufc/58963
Type: Artigo de Periódico
Title: A trypsin inhibitor purified from Cassia leiandra seeds has insecticidal activity against Aedes aegypti
Authors: Dias, Lucas P.
Oliveira, Jose T. A.
Rocha-Bezerra, Lady C. B.
Sousa, Daniele O. B.
Costa, Helen P. S.
Araújo, Nadine M. S.
Carvalho, Ana F. U.
Tabosa, Pedro Matheus Sousa
Monteiro-Moreira, Ana C. O.
Lobo, Marina D. P.
Moreno, Frederico B. M. B.
Rocha, Bruno A. M.
Lopes, José L. S.
Beltramini, Leila M.
Vasconcelos, Ilka M.
Keywords: Cassia leiandra;Protease inhibitor;Kunitz inhibitor;Insecticidal activity;Aedes aegypti;Biocontrol
Issue Date: 2017
Citation: DIAS, Lucas P. et al. A trypsin inhibitor purified from Cassia leiandra seeds has insecticidal activity against Aedes aegypti. Process Biochemistry, [s. l.], v. 57, p. 228–238, 2017.
Abstract: A trypsin inhibitor from Cassia leiandra seeds, named ClTI, was purified, characterized, and its insecticidal activity against Aedes aegypti evaluated. ClTI was purified by DEAE-Cellulose and trypsin-Sepharose 4B chromatography, with a 15.5-fold purification and 2.4% yield. ClTI is composed of a 19,484 Da polypeptide chain as revealed by mass spectrometry, it is not a glycoprotein, its amino acid sequence is similar to other Kunitz-type inhibitors, and it comprises 35% β-sheets, 14% β-turns, and 50% disordered secondary structures. ClTI is an uncompetitive inhibitor of bovine trypsin (IC50 of 33.81 × 10−8 M, Ki of 6.25 × 10−8 M) stable over a broad range of pHs (2.2–10.0) and temperatures (30–70 °C), but dithiothreitol led to a partial loss of the inhibitory activity. ClTI, at 4.65 × 10−6 M, reduced in 50% the activity of the Ae. aegypti midgut proteases. ClTI also promoted acute toxicity on the 3rd instar larvae of Ae. aegypti, with an LC50 of 2.28 × 10−2 M. Moreover, it caused a 24-h delay of the larvae development and 44% mortality after ten days of exposure. Altogether, these results suggest that ClTI has potential as a natural compound to control Ae. aegypti, a vector of several infection diseases.
URI: http://www.repositorio.ufc.br/handle/riufc/58963
ISSN: 1359-5113
Access Rights: Acesso Aberto
Appears in Collections:DBIO - Artigos publicados em revista científica

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