Vírus do mosaico severo do caupi-CPSMV como molécula carreadora para a p28 do vírus da artrite-encefalite caprina-CAEV

Abstract

Caprine arthritis-encephalitis virus (CAEV)

belongs to Retroviridae family, Lentivirus genus. This virus

infects caprine all over the world causing arthritis, encephalitis,

mammitis and progressive emaciating. This research showed

chimera’s building made by mixing up CAEV p28, with

glutaraldehyde, and CPSMV, purified through the

chromotography in biogel and sephadex (G-150). After that,

some measures in a spectrophometric were developed to

absorbance at 280nm. Peaks, which contained chimera, were

collected and submitted to SDS-PAGE, evidencing the band

relative to itself. Groups of swiss mice were immunized with

chimeric virus, purified CPSMV and with p28 protein using

incomplete Freund Adjuvant. CPSMV and p28 specific

antibodies recognized chimeric protein in Western Blotting

and ELISA showing the efficacy of the method. The results

showed the covalent coupling between CPSMV and CAEV

p28 was successfully archieved, originating a stable molecule,

which no disestablished the capside from CPSMV. Besides, it

showed that chimeric virus presents more immunogenicity

than protein p28 isolated. It’s suggesting CPSMV can be used

as a carrier molecule in the production of vaccines to the

virus, which infect animals.