Redução da reação oxidativa sistêmica e hepática ocasionada por estresse crônico moderado imprevisível em ratos tratados com eletroacupuntura e trans-anetol

Abstract

Prolonged exposure to stress is one of the main factors that have been associated with the progression of pathological processes related to oxidative pathways that lead to depression. Based on the demonstration that trans-anethole and electroacupuncture alone have modulating effects on the production of anti-oxidative enzymes and reduction of inflammatory factors, this study aims to evaluate the effect of electroacupuncture (100Hz) and trans-anethole as adjuvant on oxidative stress induced by chronic unpredictable mild stress (CUMS) from hematological and hepatic parameters. This was a prospective, controlled and comparative study using 42 healthy male rats (Rattus norvegicus: var.albinus, Rodentia, Mammalia), approved by the Animal Ethics Committee of the Ethics and Research Committee (CEPA), from the Department of Physiology and Pharmacology of the Federal University of Ceará (UFC) for the use of experimental animals under No. 21/2016. The animals were subjected to CUMS for six (6) weeks, for the development of depression-like condition, verified by the Sucrose Preference Test ,followed by 12-day treatment with Saline (G1), 100 Hz Electroacupuncture on VB34, E36 and R3 for 15 minutes on alternate days (G2), Trans-Anethole 1.0 mg / kg / day by gavage daily (G3) and Combination therapy with 100 Hz electroacupuncture and trans-anethole (G4). Blood and liver samples were collected for biochemical analysis of serum glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT), hepatic serum and hepatic myeloperoxidase (MPO), serum and hepatic TBARS, reduced serum and hepatic glutathione (GSH). The animals presented anhedonia confirmed by the Sucrose Preference Test with a significant reduction of 60% (37,75 ± 4,77) in preference to sucrose. CUMS impacted weight gain by 39,6%, and treatment promoted an average increase of 19,2%. As for the biochemical parameters, there is a reduction in GOT and GPT only with the joint therapy of 32.46% (53.27 ± 7.73) and 14.37% (45.09 ± 4.09), respectively, without significance. There was a significant reduction on MPO by G2- 39,87% (4,87 ± 0,79), G3 - 60,0% (3,25±0,74) and G4 - 78,76% (1,72 ± 0,50), as well as on plasm TBARS by reduziu G2- 39,13%(0,14 ± 0,04), G3 - 60,86% (0,09 ± 0,02) and G4- 91,30% (0,02 ± 0,01), accompanied by reduction in liver TBARS by G2 - 46,51%(0,24 ± 0,04), G3- 55,81%(0,19 ± 0,03) and G4 - 74,41% (0,12 ±0,02). On the other hand, glutathione seric increased by G2- 59,33%(105,94 ± 3,25), G3 - 121,92%(140,39 ± 4,89) and G4- 228,09%(216,32 ± 4,73) like liver increase of this substance by G2-21,67%(105,06±3,25), G3 - 40,05%(140,39 ± 4,89) and G4 -90,04% (216,32 ± 4,73). It is concluded that the treatment with electroacupuncture (100Hz) and / or anethole in animals subimetted a CUMS promotes local and systemic protection against oxidative stress, besides reducing the general inflammatory state. In addition, combination therapy had a cumulative effect showing a potentiating effect when compare to individual treatments, being a promising alternative as a therapeutic intervention in depression.