Avaliação do polimorfismo (rs10887800) do gene da renalase (RNLS) em gestantes com pré-eclâmpsia

Abstract

Introduction: Despite advances in obstetric medicine, the pathogenesis of preeclampsia (PE) remains poorly understood, it has been suggested that it results from a state of sympathetic hyperactivity, with circulating catecholamines being increased in this condition. A new enzyme, called renalase, has recently been identified and exhibits action on catecholamine metabolism and blood pressure reduction when administered in vivo. Thus, the objective of this study was to evaluate the possible relation of the polymorphism (rs10887800) of the renalase gene (RNLS) in the mechanisms that control the pathogenesis of preeclampsia. Material and Methods: This is a cross-sectional, quantitative, case-control study of 94 pregnant women with pre-eclampsia (cases) and 97 normotensive pregnant women (controls). A standardized form was used to collect demographic and clinical data, as well as the collection of oral scrapings, DNA extraction and subsequent real-time polymerase chain reaction (PCR) to evaluate the genetic polymorphism (rs10887800) in the renalase gene (RNLS). Results: There was a significant statistical difference in terms of age (p = 0.034), gestational age (p = 0.013) and BMI (p = 0.010) when compared to the PE and control groups. As expected, the means of SBP (PE 158.15 ± 1.85 vs control 113.37 ± 17.42, p = 0.013) and DBP (PE 102.19 ± 1.37 vs control 74.02 ± 0, 86, p <0.001) presented a statistically significant difference between the PE and control sgroups. Regarding the biochemical and hematological parameters, there was a significant difference for the values of platelets (p = 0.017) and uric acid (p = 0.003), comparing the groups of mild and severe PE. In terms of genotypic distribution and frequency of alleles, there was no significant association of the rs10887800 polymorphism of the RNLS gene with the development of PE and with the severity. However, the GG genotype was associated with a trend of higher risk of the disease (GG vs AG + AA: OR = 2.16, 0.97-4.86, p = 0.05). Conclusion: The rs10887800 polymorphism of the renalase gene can not be considered as a predisposing factor for susceptibility to PE in a population in northeastern Brazil, nor is it associated with its severity. However, these findings can not be generalized and it is recommended to carry out future research on larger and more extensive samples.