Atividade celulolítica e amilolítica de cepas de actinobactérias de região semiárida

Abstract

Actinobacteria are cosmopolitan Gram-positive bacteria, which have in soil their main reservoir. These microorganisms perform relevant ecological functions, such as therecycling of carbohydrates: cellulose and starch. Considering its ecological role, the objective of this work was to evaluate the amylolytic and cellulolytic activity of 22 strains of actinobacteria from soil samples from the northeastern semi-aridregion, located in Quixadá, State of Ceará. The synthesis of hydrolytic enzymes wasverified through enzymatic diffusion in specific medium andmeasurement of the hydrolysis halo of the substrate and its respective colony. The enzymatic index was determined by the ratio of hydrolysis halos. On the studied strains, all 22 were positive amylase, with emphasison AQ10, while 16 strains (75%) for a cellulase, with emphasison AQ2. These results indicate the ecological and biotechnological potential of the actinobacteria of the soil of the studied region as producers of the enzymes amylase and cellulase.