Seleção de meio de crescimento apropriado para o desenvolvimento de tubo polínico em pólen de mamoneira (Ricinus communis L.) e comparação dos perfis de proteínas de diferentes estágios de desenvolvimento do pólen mediante eletroforese em gel de poliacrilamida

Abstract

The Ricinus communis L. is species, member of the Euphorbiaceae , is an important oilseed used mainly for biodiesel production , which explains the different studies about the biochemical characteristics of differen t tissues obtained from the plant Castor pollen represents a reproductive structure of significant importance for research relating to the formation and expansion of the cell wall, since the mechanism responsible for elongation of the pollen tube to lead the male gamete to female gamete, to allow fertilization. The pollen of this species is classified as tricolporate because it presents colpi easily identifiable by optical microsco py , and has two well defined la yers, the exine and intine. In this study was performed to determine the most efficient means of growth in the germination of castor pollen using two growth media to compare the percentage of pollen germination by counting method in a Neubauer chamber. The first medium (SB) comprised different concen trations of sucrose (2.5%, 5.0% and 7.5%), keeping constant the concentration of boron (5.0 mg / L), while the se cond medium (PGM) with sucrose, and boron, in various concentrations, but added salts. I t was promoted the ex traction of the proteins in soluti on with acetone, TCA (10.0%) and DTT ( Then, the quantification and electrophoresis in SDS PAGE of samples was performed. An optical imaging densitometry analysis program was used to graphically compare the distribution of the profiles of protein ban ds present in the resulting electrophoresis gels. Among the media examined, the middle PGM was selected as the most efficient for the germination of castor pollen, as had the highest percentage of germination. The quantification values for the resulting samples of protein extract from 1.0 mg MS pollen showed the largest protein content in pollen samples were not seeded and lower in samples of po llen with pollen tube elongate indicating the protein consumption during the pollen tube phase of elongation Th e analysis of profiles of proteins present in the gels was allowed distinction inferred that as the composition of proteins present at different stages of devel opment of pollen , since the existence of specific bands of each phase was Keywords