Avaliação da associação entre agentes biomodificadores naturais e um agente remineralizante de colágeno dentinário.

Abstract

In the search for better mechanical properties of dentin collagen, biomedific agents have been gaining a lot of attention, among them proanthocyanidin (PAC) and epigallocatechin 3 gallate (EGCG) because they have the ability to promote crosslinking and inhibit collagenolytic enzymes action . Thus, remineralizing agents such as the beta phosphate tricalcium ( β TCP) that have ability to preserve collagen, inhibiting its degradation. The aim of the present study was to evaluate the association between natural biomodorating agents and dentin collagen remineralizing agents effects on hybrid layer . Healthy human thi rd molars were transform ed in to dentin bars for evaluation of biomedical potential and remineralization capacity, as well as the medium dentin blocks for Union Strength evaluation μ TSB] (n = 6), Divided accordingly with the treatments in dentin with dist illed water (CN); epigallocatechin 3 gallate (0.1% EGCG); proanthocyanidin (PAC 6.5%); beta phosphate tricalcium ( β TCP 10%); epigallocatechin 3 gallate associated with beta tricalcium (0.1% EGCG + 10% β TCP) and proanthocyanidin associated with beta phospha te tricalcium (PAC 6.5% + 10% β TCP). The evaluation of the biomodifier potential was performed through the 3 point flexural test to measure elastic modulus (ME) (n = 10) and mass variation (MV) (n = 10) using a universal mechanical testing and precision an alytical balance; μTSB through a universal mechanical testing machine and evaluation of the remineralizing potential (n = 2) by Fourier transformer infrared spectroscopy (FTIR), the treatment application time was 1 minute. The ME and the MV were submitted to the Kolmogorov Smirnov normality test, after a two factor ANOVA test by repeated measurements, for the cases where differences were detected, Tukey's post test was performed (p <0.05 ). For the union resistance test, a one way ANOVA test was performed. In ME, it was observed that there was statistical difference in all the groups that received treatment after 14 days of aging, different from the control group. No statistical differences were observed between groups when mass variation was analyzed, as we ll as in union strength. Peak formation was observed between 3000 3500 cm 1 in all groups that were treated with polyphenols, representing the formation of ( OH) linkages. Only the PAC group 6.5% + βTCP 10% presented increase of the peak between 1000 1100 cm 1, referring to the presence of phosphate. It is concluded that the treatments used are potentially viable to enhance the mechanical properties of collagen, allowing both the formation of cross links and the deposition of mineral in the collagen matrix.