Pontos quânticos de Carbono como uma plataforma emergente para a detecção de proteínas

Abstract

A new strategy for the detection and identification of proteins was studied using carbon quantum dots. These were prepared by the top-down method by means of low-cost synthesis by incomplete carbonization. The prepared CQDs showed sp2 hybridization, uniformity in size and strong fluorescent activity. This material was used as sensing elements to create an array of fluorescent sensors. A system containing the precursor solution of the nanoparticle was prepared by adding aliquots of solutions of metal salts with final concentration solution of 30M Cu2+ and Ni2+ concentration of 20M for Fe3+. In the presence of proteins the CQDs-M system (Metal= Fe3+, Cu2+ and Ni2+) had altered fluorescence, producing distinct fluorescence response patterns for each sample. This alteration was followed by linear discriminant analysis (LDA), where four proteins (BSA, Hb, Mb and Cit C) in a concentration of 50 nM were successfully discriminated. This work demonstrates that a protein detection matrix based on carbon-metal nanoparticle systems has high potential for medical diagnostic applications.