Fracionamento bioguiado do extrato bruto da esponja marinha monanchora arbuscula (duchassaing & michelotti, 1864) coletada no parque estadual marinho pedra da risca do meio — Ce

Abstract

Marine sponges have gained great importance as a promising source of natural products with interesting biological activities, including cytotoxicty. This work aimed at the chemical fractionation of the extract derived form the marine sponge Monanchora arbuscula collected at the Pedra da Risca do Meio Marine State Park - CE bio-guided by cytotoxic activity. The collected material was homogenized and concentrated as a crude extract. The extract was partitioned with different solvents and fractionated in chromatographic columns. The compounds Ptilocaulin (PT), 8b-Hydroxyptilocaulin (8HPT), Mirabilin B and the epimeres mixture of 1,8a;8b,3a-didehydro-8[3- hydroxyptilocaulin and 1,8a;8b,3a-didehydro-8a-hydroxyptilocaulin were obtained from the active fractions, dichioromethane and ethyl acetate. A mixture of yet unidentified alkaloids, was also obtained. For cytotoxicity evaluation, the MTT colorimetric method was used, as well as four different tumor cell lines - HL-60 (leukemia), HCT- 8 (colon), MDA-MB-435 (melanoma) and SF-295 (glioblastoma) - during a 72h incubation period. The active samples were evaluated for lytic effects on Mus musculus mouse erythrocytes. PT and 8HPT showed a cytotoxic activity towards all tumor cell lines tested, exhibiting a relative specificity to the melanoma and leukemia cell lines, with an average of 1.8-fold greater potency of PT compared to 8HPT. These compounds also showed hemolytic activity, but in concentrations much higher than those obtained for mean antiproliferative activity on the tumor cell lines.