Associação entre a técnica úmida etanólica e diferentes agentes de biomodificação de colágeno no procedimento adesivo em dentina

Abstract

The technique Ethanol wet-bonding (EWB) and the use of dentin biomodifiers have emerged as strategies to minimize the loss of restorations through hydrolytic and enzymatic action, however this has been used separately. The objective of the present study was to evaluate the influence of the association of biomodifiers with the ethanolic wet technique on the mechanical properties of the collagen at different application times, as well as its bond strength and degree of conversion in the adhesive procedure on dentin substrate. In order to evaluate the degree of conversion in situ [DC] (n = 3), microtensile bond strength testing [μTSB] was used to evaluate the biomidifacodor potential, both in the form of dentin bars, (n = 6) and Nanoleakage evaluation [NE] (n = 2), the groups were divided according to the treatments in dentin with distilled water control (WC); absolute ethanol (100% absolute ethanol, Cequimica, Fortaleza, Brazil) (AE); (90% PACs, Vitis vinifera, Gold Mega-Natural, polyphenols, Madera, USA) (PAC + AE), with the incorporation of proanthocyanidins (PACs) 6.5ppp% of grape extract; absolute ethanol with the incorporation of 0.3 M 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (powder, EDC Sigma Aldrich, St. Louis, USA) (EDC + AE); absolute ethanol with the incorporation of 5 w / w% Glutaraldehyde (25% Sigma Aldrich, St. Louis, USA) (GA + AE). The evaluation of the biomodifier potential was carried out using a 3-point flexural test to measure elasticity modulus (ME) (n = 10) and mass change [MC] (n = 10) using a universal mechanical tests and precision analytical balance, for these tests were used different times of immersion of the treatments; GC in situ, with the aid of a micro-raman spectrometer; μTSB through a universal mechanical testing machine and NI by means of scanning electron microscopy. In the DC, μTSB and NE tests the treatment application time was 1 minute. For the ME an analysis of variance was used for two criteria, in the VM the Kruscal-Wallis no parametric test was used in the DC and μTSB was used a variance analysis, for all the quantitative analyzes a level of significance of 5% and in nanoinfiltration a qualitative analysis was used. In the ME, it was observed that there was a statistical difference in the different application times for all groups (p≤0.001), except for the PAC + EA that has equivalence with both 1 minute and 1 hour of immersion. All of these treatments did not present statistical difference in DC in situ (p = 0.529) and µTSB (p = 0.194). In the qualitative analysis, large areas of crack formation were observed in this region in the EA group. It was concluded that the association between the TUE technique and the use of biomedical agents will improve the mechanical properties of the collagen, but it has not been shown to influence the adhesive procedure.