Estudo das alterações morfológicas dos neurônios do sistema nervoso entérico no modelo experimental de ileíte intestinal induzidos por diferentes cepas de Clostridium difficile

Abstract

Clostridium difficile is a gram bacillus that is positively responsible for nosocomial diarrhea associated with the use of antibiotics. It can cause pseudomembranous colitis leading to death. C. difficile has promoted a study on new strains and their virulence, by our study group, through patients with diarrhea, monitored at Haroldo Juaçaba Hospital (HHJ) of the Ceará Cancer Institute (ICC), in order to identify the C. difficile and characterize as strains found. The study of the virulence of C. difficile strains isolated in the HHJ is through the injection of culture supernatants of the bacteria into ileal loop of Swiss male mice weighing between 25-30 g, randomly divided into 4 groups, two controls : PBS (negative control); ATCC 700057 (nontoxigenic strain), and 2 experimental NAP1 (hypervirulent strain) and ICC 01. This latter strain was isolated in faecal samples from patients admitted to the ICC. The animals were submitted to the ileal loop model followed by the injection of the supernatant. Four hours after the surgical procedure, sacrificed animals, ileal loop, plague and measurement and aliquotting for the tests: routine hematoxylin and eosin technique to evaluate the histopathological parameters by scores, immunohistochemistry and count of neurons and glial cells by Microscope Confocal. The mice exposed to the supernatant of the NAP1 and ICC 01 strains had a higher intensity of inflammatory response verified by a greater intensity of histopathological alterations such as desquamation, edema, infiltration and hemorrhage in relation to the controls, also presented a decrease in the density of NOS neurons.