Avaliação da toxicidade e dos efeitos antinociceptivo e antiinflamatório na ATM de ratos de derivados semissintéticos de um benzilisotiocianato isolado de moringa oliefera Lam.

Abstract

Inflammation is an important clinical condition that affects the temporomandibular joint (TMJ), but the mechanisms are not fully understood and there is no effective therapy for this clinical condition. Moringa oleifera Lam has analgesic and anti-inflammatory activities that can be attributed to their isothiocyanates . Objectives: The objectives of this study are verify therapeutic application (toxicity, antinociceptive and antinflammatory effectiveness) in rats TMJ of a isothiocyanate from Moringa oleifera Lam and their 7 derived semi-synthetic. Materials and methods: The toxicity of substances in mice (male and female) was evaluated for 14 days with the doses MC-1 1μg/kg; MC-D1 1μg/kg; MC-D3 100μg/kg; MC-D6 1μg/kg; MC-D7 1μg/kg; MC-D8 1μg/kg; MC-D9 10mg/kg; MC-H 1μg/kg. In substances considered safe (MC-D7, MC-D9 and MC-H) was determined to antinociceptive efficacy by Formalin test (1.5%, 50 μL) and Serotonin test (255mg), by intra-articular injection (i.a.) in the left TMJ of rats. The anti-inflammatory effect was assessed by vascular permeability with Blue Evans dye. Also it was performed the Rotarod test to assess effects on motor function. Results and Discussion: Among the derivatives only tested MC-D7, MC-D9 and MC-H showed no toxicity, evidenced by survival rate, biochemical and histological analysis. These derivatives have reduced the formalininduced inflammatory hyperalgesia but only MC-H reduced the hyperalgesia induced by serotonin, suggesting action on adrenergic receptors. These 3 derived also reduced significantly the plasma extravasation in Blue Evans dye test, suggesting antiinflammatory activity. In the Rotarod test there were no differences between the tested groups and control, reinforcing the hypothesis of analgesic effect, but not on the motor skills. Conclusion: Derivatives MC-D7, D9-MC and MC-H were effective in reducing the hyperalgesia induced by formalin in the TMJ of rats. New research is necessary to investigate the mechanisms of action of these drugs.