Análise da resposta humoral contra H. pylori em pacientes portadores e não portadores de câncer gástrico

Abstract

Helicobacter pylori have accompanied humanity in all its migration routes across the planet. Being the cosmopolitan H.pylori bacteria that evolved and adapted the most adverse conditions through mutation and genetic exchange that resulted in favorable changes morph physiological. Such changes contributed to the development of highly virulent strains of bacteria which cause different pathophysiological conditions in the host. The high genetic variability of H. pylori hinders research to uncover associations with certain gastrointestinal conditions. However, bacterial virulence markers have been identified and associated with different gastrointestinal diseases. H. pylori strains that express proteins Cag A and Vac A has been found in patients with gastric cancer, others expressing Dup A and Oip A are found in patients with gastric ulcer were considered protective genes, since they are not related with development of gastric cancer. The methods used to detect H. pylori infection can be divided into invasive and non-invasive. The endoscopy is an invasive method is where the biopsy urease test to detect the presence of bacteria. The problem is that endoscopy can’t identify discrete points of colonization of H. pylori, or else, is indicated when the patient is already symptomatic, where you have a situation of advanced gastric epithelial cell injury. Among the non-invasive methods have serological methods. Among these serological methods have immunoblotting method that searches the presence of specific antibodies (Ig G). The antibodies against virulence factors and specific proteins (Cag A, Vac, Ure A, Ure B, and flagellar protein) serves both to detect infection and measure the type strain of H. pylori present in the infected individual. This method can be used to investigate the humoral response in patients with and without gastric cancer infected with H. pylori.