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http://repositorio.ufc.br/handle/riufc/9472
Tipo: | Artigo de Periódico |
Título : | Glutamine and ornithine alpha-ketoglutarate supplementation on malate dehydrogenases expression in hepatectomized rats |
Autor : | Guimarães Filho, Artur Cunha, Rodrigo Maranguape Silva da Vasconcelos, Paulo Roberto Leitão de Guimarães, Sergio Botelho |
Palabras clave : | Glutamina;Hepatectomia;Regeneração Hepática |
Fecha de publicación : | jun-2014 |
Editorial : | Acta Cirúrgica Brasileira |
Citación : | GUIMARÃES FILHO, A. et al. Glutamine and ornithine alpha-ketoglutarate supplementation on malate dehydrogenases expression in hepatectomized rats. Acta Cirúrgica Brasileira, São Paulo, v. 29, n. 6, jun., 2014. |
Abstract: | PURPOSE: To evaluate the relative gene expression (RGE) of cytosolic (MDH1) and mitochondrial (MDH2) malate dehydrogenases enzymes in partially hepatectomized rats after glutamine (GLN) or ornithine alpha-ketoglutarate (OKG) suplementation. METHODS: One-hundred and eight male Wistar rats were randomly distributed into six groups (n=18): CCaL, GLNL and OKGL and fed calcium caseinate (CCa), GLN and OKG, 0.5g/Kg by gavage, 30 minutes before laparotomy. CCaH, GLNH and OKGH groups were likewise fed 30 minutes before 70% partial hepatectomy. Blood and liver samples were collected three, seven and 14 days after laparotomy/hepatectomy for quantification of MDH1/MDH2 enzymes using the real-time polymerase chain reaction (PCR) methodology. Relative enzymes expression was calculated by the 2-ΔΔCT method using the threshold cycle (CT) value for normalization.RESULTS: MDH1/MDH2 RGE was not different in hepatectomized rats treated with OKG compared to rats treated with CCa. However, MDH1/MDH2 RGE was greater on days 3 (321:1/26.48:1) and 7 (2.12:1/2.48:1) while MDH2 RGE was greater on day 14 (7.79:1) in hepatectomized rats treated with GLN compared to control animals. CONCLUSION: Glutamine has beneficial effects in liver regeneration in rats by promoting an up-regulation of the MDH1 and MDH2 relative gene expression. |
URI : | http://www.repositorio.ufc.br/handle/riufc/9472 |
ISSN : | 1678-2674 Online |
Aparece en las colecciones: | DCIR - Artigos publicados em revista científica |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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2014_art_sbguimaraes.pdf | 245,49 kB | Adobe PDF | Visualizar/Abrir |
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