Avaliação da sensibilidade de Candida parapsilosis à caspofungina utilizando solução de albumina 50%

Abstract

The treatment of fungal infections is limited, because unlike antibiotics, there are several options, the available antifungal agents are few. Moreover, the fungal resistance further limits the use of some antifungals. The echinocandins are the most recent drugs used to treat systemic fungal infections. Caspofungin is the prototype drug of this group, which has a similar mechanism to the other echinocandins. It is active against strains of Candida spp. and Aspergillus spp. Albumin is the most abundant, multifunctional, negatively charged, plasma protein, which has antioxidant properties, carriers and enzymatic functions. This protein serves as an endogenous drug carrier and exerts an effect on the Minimum Inhibitory Concentration (MIC) of caspofungin. This study evaluated the influence of bovine serum albumin 50 mg/m on the values of MICs for Candida parapsilosis. The effect of albumin was measured by broth microdilution method in RPMI. 22 strains of C. parapsilosis were tested. When caspofungin was evaluated alone was observed that all C. parapsilosis showed sensitivity. A bovine albumin solution 50 mg/ml increased the MICs of caspofungin. The effect remained significant after 24 h and 48 h incubation. The MICs of caspofungin obtained with albumin were statistically different MICs without albumin. The elevation of the MIC was about 128 times. The clinical effects of this increase are not yet known and this interaction is not completely understood and studies with larger numbers of strains are needed to elucidate and understand the implications of this phenomenon.