Avaliação do perfil bioquímico de camundongos sedentários em uso de suplementação com creatina

Abstract

The use of nutritional supplements has been indiscriminate. Among these, the only one who has a proven ergogenic effect is creatine bound to training, is the most used to increase muscle mass. Creatine is obtained through the diet or endogenous synthesis from amino acids. This compound provides energy in the phosphate forms, from creatine phosphate to ADP, forming ATP. In Brazil, the use of creatine is regulated by RDC No. 18/2010. This legislation comes creatine, as a product intended to complement the endogenous creatine stock and claims that use of the supplement should follow these recommendations: The ready-to-eat product must contain from 1.5 g to 3.0 g of creatine per serving; The creatine monohydrate with a degree of purity of at least 99.9%, and others must be used. Thus, the present study aims to evaluate biochemical parameters (ALT, AST, UREA, creatinine, total cholesterol, triglycerides and glucose) of mice submitted to oral supplementation with creatine. For this, they used 40 male mice divided into groups (n=10): negative control (NC), creatine 1 (CR1), creatine 2 (CR2) and creatine 3 (CR3). The CN group received standard chow and water, while the other groups CR 1, CR 2 and CR 3 underwent the following doses respectively, 25 g/day/animal, 100g/day/animal and 150g/day/animal for seven days. All doses were calculated based on the RDC 18/2010 and the actual dose used by athletes. After this period, blood collection was performed. Then, were administered to the animals in each test group, maintenance doses of creatine (25g/day/animal) for 28 days. Next, another blood sample was collected. All values were expressed as mean standard error (EPM) and to compare the means used to ANOVA followed by Tukey's post-test (p <0.05). Thus, it was observed that glucose levels increased 46.25%, 43.75% and 28.3% in the groups CR1, CR2 and CR3, respectively, compared to the CN group during overcharging. Have total cholesterol levels, triglycerides and liver enzymes have not changed. In the analysis of kidney function, decreasing the urea had 19.27% at the highest dose during overcharging and creatinine increased 228.57%, 181.00% and 90.42% in the groups CR1, CR2 and CR3, respectively, compared to the negative control in overhead. In the maintenance, creatinine levels increased 43.65% and 42.55% in CR2 and CR3, respectively, compared to the CR1 group. Thus, it can be seen that creatine may promote significant biochemical changes, especially when used in high doses.