Avaliação da atividade anti-inflamatória e antioxidante de bioprodutos obtidos de Amburana cearensis

Abstract

Amburana cearensis is a typical wild plant in the Northeastern Brazil, known as “cumaru” and popularly used in the form of “lambedor” for the treatment of respiratory diseases. Chemical studies isolated several substances from plant trunk bark (CANUTO, 2006), such as coumarin (CM) and amburosídeo A (AMB), majoritarian principles used as markers for the standardization of its dry extract (HPLC-DAD) (ARARUNA, 2013). Pharmacological studies showed anti-inflammatory activity, anti-nociceptive, muscle relaxant, antiedematogenic and antioxidante of non-standardized extract and some isolated compounds from the plant (Leal, 1995, 1997, 2000, 2003). Faced with the potential of A. cearensis the aim of the present study was to evaluate the anti-inflammatory and antioxidant activity of standardized dry extract (ESAC) and the phenolic fraction (FFAC) of A. cearensis in human neutrophils. It was initially evaluated the effects of ESAC and FFAC measured on neutrophil degranulation by the release of MPO enzyme was reduced by ESAC (25, 50 and 100 ug / ml) up to 75.26% and the FFAC (25, 50 and 100 ug / ml) up to 89.88% effect comparable to indomethacin, the standard drug which reduced the release of MPO in the 76.12 and 70.45%, respectively. To evaluate the effects of ESAC and FFAC on the oxidative metabolism of neutrophils was performed chemiluminescence assay (luminol probe) for the quantification of reactive oxygen species (ROS) produced by neutrophils activated by PMA. Any ESAC as FFAC reduced the production of oxidants, especially via H2O2-MPO-HOCl system with maximum inhibition of 54.81% and 63.17%, respectively. In the assessment of cytotoxicity of these derivatives (MTT and LDH activity test), the absence of cytotoxicity of these can be seen, confirming that the anti-inflammatory effect is determined not related to a toxic effect. Based on these results, both the ESAC as FFAC have anti-inflammatory and antioxidant activities by modulating pro-inflmatórios mechanisms of human neutrophils, cellular targets of interest in the development of anti-inflammatory drugs