Identificação de espécies de Candida por PCR em tempo real em amostras cérvico-vaginais de citologia em meio líquido com achados de Candida SP

Abstract

Among the most frequent complaints from women seeking gynecology services is vaginal discharge. Vulvovaginal candidiasis is one of the main diagnoses in this situation. It is caused by fungi of the genus Candida that inhabit the normal microbiota of the vagina, but when there is an imbalance in this microbiota or the host's immune system is compromised, the yeasts tend to proliferate and have clinical manifestations. Candida sp is the fungus most frequently identified, by morphological methods, in cases of vaginitis. It is necessary to carry out complementary laboratory tests for effective treatment. In addition, it is essential to identify the most common species among women treated in our country. Therefore, the objective of this study was to identify Candida species by Multiplex PCR, in cervical-vaginal samples from women with liquid cytology findings. This is a cross-sectional, descriptive and quantitative study, carried out with 95 cervical-vaginal samples from women with Candida sp findings on cytology, treated in private services, between January and July 2022 in the city of Fortaleza. The analysis of study variables was carried out using the GraphPad Prism® 6.0 software. Candida albicans was the most detected species with a prevalence of 62.11%. It is concluded that the use of the Multiplex PCR test is a viable alternative for identifying Candida species, since the tested species were detected, mainly Candida albicans.